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. 2021 Apr 22;16(5):1210–1227. doi: 10.1016/j.stemcr.2021.03.031

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Characterization of gene expression and differentiation trajectory in gastruloid EPI-like and mesoderm clusters

(A) UMAP of untreated cells at 0 h and gastruloid EPI-like cells at 12, 24, and 44 h.

(B) Expression of the indicated markers in the indicated cell types over the gastruloid time course.

(C) Diffusion maps and pseudotime plots of gastruloid EPI-like and CS7 EPI and PS.

(D) Expression of the indicated PS and mesoderm markers in gastruloid mesoderm-1 and -2 clusters over the time course.

(E) Diffusion maps showing the arrangements of gastruloid EPI-like and mesoderm-1/2, and CS7 EPI, PS, and nascent/emergent mesoderm. (Top left) Gastruloid cells shown with CS7 cells in the background, (top center) CS7 cells shown with gastruloid cells in the background, and (top right) a pseudotime plot of both gastruloid and CS7 cells combined. (Bottom) Expression of the indicated posterior EPI and mesoderm markers in gastruloid and CS7 cells along the EPI-to-mesoderm transition.