Figure 6.

Characterization of gene expression and differentiation trajectory in gastruloid endoderm

(A) Expression of endoderm and anterior PS markers.

(B) Diffusion maps showing arrangements of gastruloid mesoderm-1/2 and endoderm, and CS7 PS, nascent/emergent mesoderm, and endoderm. (Top left) Gastruloid cells shown with CS7 cells in the background, (top center) CS7 cells shown with gastruloid cells in the background, and (top right) a pseudotime plot of both gastruloid and CS7 cells combined. (Bottom) Expression of the indicated mesoderm and endoderm markers in gastruloid and CS7 cells along PS-to-mesoderm and PS-to-endoderm trajectories.