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Identification of ROCK1 binding proteins using coimmunoprecipitation in combination with mass spectrometry-based proteomic analysis. (A) GO annotation of the identified proteins, which were enriched in different terms. (B) Deepened analysis of GO annotation revealed significant enrichment of the term ‘protein binding’. (C) Differentially expressed proteins were primarily clustered in three complexes. GO, Gene Ontology; ROCK1, Rho-associated protein kinase 1.
