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. 2021 Jun 2;46(1):151. doi: 10.3892/or.2021.8102

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — ROCK1 and DCTN2 form a protein complex in HCT116 and IEC-6 cells. (A and B) ROCK1 antibody pulled down DCTN2, which was enhanced by the experimental treatment. (C and D) DCTN2 antibody pulled down ROCK1, which was enhanced by the experimental treatment. Lower band below DCTN2 is likely to be the IgG used for IP, which was visualized by the secondary antibody used during western blot analysis. **P<0.01 vs. control. C, control sample; T, treated sample; IP, immunoprecipitation; ROCK1, Rho-associated protein kinase 1; DCTN2, dynactin subunit 2.