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. 2021 Jun 2;46(1):151. doi: 10.3892/or.2021.8102

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Disruption of the ROCK1-DCTN2 protein complex inhibits centrosome amplification in HCT116 cells. siRNA-knockdown decreased the expression levels of (A and B) ROCK1 and (C and D) DCTN2. (E and F) Individually, knockdown of ROCK1 and DCTN2 partially attenuated centrosome amplification. (G and H) Co-transfection with ROCK1 and DCTN2 siRNAs inhibited centrosome amplification to a considerable degree. In total, 200 cells used for centrosome quantification. Percentage of centrosome amplification=(the number of cells with centrosome amplification/200) ×100. **P<0.01 vs. control; ^##P<0.01 vs. samples treated high glucose, insulin and palmitic acid. Glu, high glucose; Pal, palmitic acid; Ins, insulin; NC, non-coding RNA; ROCK1, Rho-associated protein kinase 1; DCTN2, dynactin subunit 2; siRNA, small interfering RNA.