Figure 2.

Perampanel relieves hemin-induced HT22 neuronal injury in vitro. (A) MTT assay of HT22 cells treated with 0–140 µM hemin. 100 µM hemin was used as the test dosage. (B) MTT assay of HT22 cells treated with hemin alone or hemin and perampanel (n=5). (C) Representative images of live (green)/dead (red) staining assay of HT-22 neurons exposed to hemin (100 µM, 72 h) in the presence and absence of perampanel (n=5). Scale bar=100 µm. Error bars indicate SEM. ^##P<0.01 vs. vehicle group; ^&&P<0.01 vs. hemin group. Per, perampanel.