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. 2021 May 16;13(6):e13074. doi: 10.15252/emmm.202013074

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© 2021 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV2 — A, B
Proximity ligation assays (PLAs; yellow) combining an anti‐VDAC1 (localizing to the outer mitochondrial membrane) and an IP₃R‐antibody (localizing to the ER) and DAPI staining of nuclei (blue) at baseline and after thapsigargin (1.0 µM, 5 h) exposure for PLNic (A) and PLN p.Arg14del (B) after transduction with control, GCaMP6f, or parvalbumin virus.

C
Negative control: Anti‐mouse, anti‐rabbit antibodies alone. Scale bar 20 µm.