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. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Mol Ther. 2005 Nov;12(5):842–851. doi: 10.1016/j.ymthe.2005.03.017

FIG. 2.

FIG. 2.

Cytotoxicity of ACE-CD/5-FC on tumor cells. (A) Structure of ACE-CD, showing location of IRES-CD insert between env and 3′ UTR. (B) ACE-CD-transduced U-87 cells were treated with 5-FC on day 0 at various concentrations ranging from 0 to 5 mM. Viability of cells was determined in a triplicate repeat with the MTS assay. The x axis shows days after 5-FC incubation. The y axis shows the viability percentage of the cells normalized to that of negative control, 0 mM. (C) The ACE-CD-transduced and untransduced U-87 cells were mixed at various initial ratios (0, 0.1, 1, 10, and 100% of ACE-CD-transduced cells) and seeded onto 96-well plates. The mixed cell populations were exposed to 2 mM 5-FC or to control medium without 5-FC, and viability of cells was determined with MTS assay. The values shown of viability percentage of the cells were normalized to that of negative control (0%/5-FC−). 5-FC−, without 5-FC incubation. 5-FC+, with 5-FC incubation.