Skip to main content
. 2021 May 25;11:640276. doi: 10.3389/fonc.2021.640276

Figure 4.

Figure 4

DLEU1 interacts with miR-320b in CRC. (A) The levels of DLEU1 in the nuclear and cytoplasm of both LoVo and SW480 cells were determined using qRT-PCR after subcellular fractionation assay, *P < 0.05, **P < 0.01 vs. cytoplasmic. (B) DLEU1 interacted with miR-320b via bioinformatics analysis. (C) DLEU1 interacted with miR-320b using dual luciferase reporter assay, **P < 0.01 vs. NC mimic. (D) The levels of miR-320b was determined using qRT-PCR, **P < 0.01 vs. sh-NC. (E) Relative enrichment of DLEU1 and miR-320b in LoVo and SW480 cells was determined using RIP assay, ***P < 0.001 vs. IgG. (F) The relative expression of miR-320b was determined by qRT-PCR in non-tumor and CRC tissues, **P < 0.01 vs. non-tumor tissues. (G) The relative expression of miR-320b was determined by qRT-PCR in CRC cell lines, *P < 0.05, **P < 0.01 vs. HIEC. (H) The correlation between DLEU1 and miR-320b was assessed using Pearson’s correlation analysis.