Figure 5.

The effects of CCL15 on malignant phenotypes of gastric cancer (GC) cells in vitro. (A) Negative control and sh-CCL15 lentivirus were transfected in MKN45 cells and CCL15 protein expression was detected by Western blot. β-actin was used as internal control. (B) MKN45 cell viability infected with lentivirus expressing shRNAs targeting CCL15 or control shRNA was examined by CCK8, and OD₄₅₀ was detected at the indicated time (**P < 0.01, n = 3). (C, D) Cell migration and invasion was performed using transwell assays. Migrated (C) or invaded (D) cells were counted by ImageJ (*P < 0.05, **P < 0.01, n = 3) (Crystal Violet staining, 20×). (E) The prognostic value of CCL15 in GC patients using tissue microarrays. (F) The prognostic value of CCL15 in GC patients of the Firehose legacy cohort using the cBioPortal database (Z-Score = 2). (G) The prognostic value of CCL15 in GC patients of the PancancerAtlas cohort using the cBioPortal database (Z-Score = 2).