. Author manuscript; available in PMC: 2021 Jun 8.

Published in final edited form as: Chem Res Toxicol. 2020 Jul 22;33(8):2197–2207. doi: 10.1021/acs.chemrestox.0c00223

Table 2.

Levels Acr-dGuo (1 and 2), εdAdo (3), and εdCyd (4) in oral rinse DNA from smokers (A) and non-smokers (B).

A. Smokers Oral rinse sample	DNA (μg) ^a	α-OH-Acr-dGuo (1)	γ-OH-Acr-dGuo (2)	εdAdo (3)	εdCyd (4)
A. Smokers Oral rinse sample	DNA (μg) ^a	adducts/10⁹ nucleotides

1	13.1	4.01	91.6	5.84	3.06
2	17.9	5.19	170	9.76	4.77
3	8.23	67.5	211	52.3	37.4
4	24.9	ND ^b	254	17.4	9.89
5	2.51	ND	ND	12.2	6.91
6	16.9	51.9	365	48.7	45.9
7	9.74	ND	10.8	9.32	736
8	6.36	5.06	1020	15.9	21.8
9	5.52	22.9	74.9	12.5	ND
10	7.31	ND	82.2	15.6	46.3
11	17.2	6.58	140	2490	15.9
12	4.23	ND	87.8	307	1510
13	4.35	14.5	167	337	791
14	6.92	8.43	82.9	39.9	387
15	22.1	4.59	51.8	ND	ND
16	30.8	18.6	170	14.8	23.6
17	11.2	7.83	14.9	45.9	31.6
18	33.5	ND	14.9	12.5	3.06
19	26.3	ND	89.1	9.69	5.35
Mean ± S.D.^c	14.2 ± 9.49	12.1 ± 17.9	163 ± 227	182 ± 568	194 ± 400

B. Non-smokers Oral rinse sample	DNA (μg) ^a	α-OH-Acr-dGuo (1)	γ-OH-Acr-dGuo (2)	εdAdo (3)	εdCyd (4)
B. Non-smokers Oral rinse sample	DNA (μg) ^a	adducts/10⁹ nucleotides

1	1.54	ND ^b	ND	ND	ND
2	5.15	ND	10.1	3.74	12.7
3	9.81	ND	10.6	6.49	5.07
4	14.3	2.11	8.66	3.86	3.83
5	11.8	3.18	13.8	3.97	4.32
6	13.1	ND	ND	11.8	10.6
7	18.1	ND	7.21	6.64	5.96
8	18.9	ND	ND	55.9	50.2
9	20.9	ND	4.29	3.79	3.41
10	18.6	ND	8.31	5.58	5.01
11	31.2	ND	1.32	1.42	ND
12	30.4	ND	4.52	2.48	ND
13	9.48	ND	5.75	7.63	ND
14	23.3	ND	3.32	3.29	ND
15	6.12	ND	5.86	5.56	ND
16	27.3	ND	0.99	1.44	ND
17	9.32	ND	ND	3.69	ND
18	5.87	ND	ND	3.95	ND
19	14.8	ND	14.5	4.84	ND
20	23.3	ND	4.84	8.58	ND
Mean ± S.D.^c	15.6 ± 8.61	1.85 ± 2.08	5.95 ± 4.23	7.69 ± 11.7	6.07 ± 10.9

calculated on the basis of the dGuo quantified by HPLC-UV

ND: not detected

calculated using ½ LOQ of the adduct for ND values