. Author manuscript; available in PMC: 2021 Jun 8.

Published in final edited form as: Chem Res Toxicol. 2020 Jul 22;33(8):2197–2207. doi: 10.1021/acs.chemrestox.0c00223

Table 3.

Levels Acr-dGuo (1 and 2), εdAdo (3), and εdCyd (4) in cytobrush DNA from smokers (A) and non-smokers (B). There is a spacing problem in part B

A. Smokers Cytobrush sample	DNA (μg) ^a	α-OH-Acr-dGuo (1)	γ-OH-Acr-dGuo (2)	εdAdo (3)	εdCyd (4)
A. Smokers Cytobrush sample	DNA (μg) ^a	adducts/10⁹ nucleotides

1	1.59	ND ^b	51.7	15.1	ND
2	2.65	38.9	1560	44.9	ND
3	1.88	ND	35.7	18.7	ND
4	1.64	ND	314	1200	nd ^c
5	2.17	ND	ND	12.1	ND
6	1.08	34.8	302	58.7	108
7	3.99	ND	15.6	9.24	ND
8	0.59	44.9	1950	58.2	ND
9	2.09	ND	146	16.3	32.9
10	0.66	ND	216	47.5	ND
11	2.44	ND	20.5	14.9	ND
12	2.86	ND	34.6	12.2	351
13	2.94	ND	83.2	9.05	14.1
14	2.16	ND	12.1	14.8	30.1
15	2.81	ND	20.8	11.9	ND
16	2.39	ND	23.5	11.8	49.2
17	3.02	ND	76.2	7.21	16.2
18	3.81	ND	29.5	10.7	ND
19	4.72	ND	19.7	6.33	ND
Mean ± S.D. ^d	2.27 ± 0.94	11.8 ± 13.1	259 ± 540	82.9 ± 271	36.9 ± 79.9

B. Non-smokers Cytobrush sample	DNA (μg) ^a	α-OH-Acr-dGuo (1)	γ-OH-Acr-dGuo (2)	εdAdo (3)	εdCyd (4)
B. Non-smokers Cytobrush sample	DNA (μg) ^a	adducts/10⁹ nucleotides

1	0.83	ND ^b	ND	ND	ND
2	2.83	ND	ND	9.19	ND
3	3.71	ND	16.5	8.91	ND
4	0.85	ND	ND	19.3	ND
5	1.76	ND	ND	11.3	ND
6	2.59	ND	10.8	14.4	33.3
7	1.31	ND	ND	11.4	1060
8	1.39	ND	ND	11.6	ND
9	1.44	ND	ND	10.7	ND
10	1.97	ND	11.3	7.41	ND
11	5.06	ND	ND	5.07	ND
12	9.52	ND	ND	4.62	ND
13	2.59	ND	ND	7.98	ND
14	3.58	ND	ND	11.4	ND
15	3.49	ND	ND	6.62	ND
16	5.26	ND	ND	5.12	ND
17	4.56	ND	ND	143	246
18	5.83	ND	ND	4.06	ND
19	2.92	ND	ND	7.69	ND
20	4.33	ND	ND	5.94	ND
Mean ± S.D. ^d	3.29 ± 2.10	ND	7.37 ± 5.09	16.2 ± 30.2	72.2 ± 238

calculated on the basis of the dGuo quantified by HPLC-UV

ND: not detected

nd: not determined

calculated using ½ LOQ of the adduct for ND values