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. 2021 Jun 7;21:298. doi: 10.1186/s12935-021-01983-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of the expanded HSCs CD34+ progenitor. A and B, the comparison of CFU assay results between the two groups treated with SFT (SCF, Flt3-L and TPO) and SFTG36 (SCF, Flt3-L, TPO, GM-CSF, IL-3 and IL-6) cocktail cytokines and morphological analysis by Wright-Giemsa staining. C–E, both expanded cell populations were analyzed using flowcytometry and cell counting on the day 7. C, Analyses of fold expansion and D, cell viability of both groups. E, the phenotypic analysis of the expanded cells for CD45, CD34 and CD45/CD34 markers are shown as flowcytometry zebra plots. The comparison of markers expressions ****P < 0.0001, indicating a statistically significant increase in the expanded SFTG36 cell population compared to SFT cell population. p<0.01 was considered significant (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001). Data are demonstrated as mean ± SD, n =4.