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. 2021 Jun 7;21:298. doi: 10.1186/s12935-021-01983-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Comparison of characteristics of differentiated NK cells. A and B assessment of CD56/CD16 and CD3 markers expression among all 4 groups (IL-7+IGF-1, IL-21+IGF-1, IL7+IL-21+IGF-1 and IL-7+IL-21+SIS3+IGF-1) showed a significant expression difference in the groups IL-7+IL-21+ IGF-1 (P < 0.01) and IL-7+IL-21+ IGF-1+SIS3 (P < 0.01). C, these 2 groups also demonstrated significant difference in the expression of NKG2D compared to the other groups. D, the expansion potential analysis showed that IL-7+IL-21+ IGF-1+SIS3 group had the highest level of Fold expansion among all groups (P < 0.05). It should be noted that all expressions were assessed in the viable cells (>90%±3.00). P<0.01 was considered significant (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001). Data is demonstrated as mean ± SD, n = 3.