Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 7;40:188. doi: 10.1186/s13046-021-01996-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Overexpression of GPD1 inhibited lipid metabolism in ccRCC cells. (A) KEGG analysis and gene ontology (GO) analysis of the differentially expressed genes (|log2FC| > 1, FDR-adjusted p < 0.05) in 786O-NC and 786O-GPD1 cells. (B) GSEA revealed that sterol biosynthetic gene sets, fatty acyl-CoA metabolism gene sets, and sterol metabolism gene sets were less enriched in 786O-GPD1 cells (FDR < 25% and p < 0.05). (C) Western blotting of indicated proteins in Caki1-NC, Caki1-GPD1, 769P-NC and 769P-GPD1 cells. (D) Images and quantifications (at 560 nm wavelength) of Oil Red O staining in Caki1-NC, Caki1-GPD1, 769P-NC, and 769P-GPD1 cells. (E) Quantitation of free fatty acids in Caki1-NC, Caki1-GPD1, 769P-NC, and 769P-GPD1 cells. Data are shown as the mean + SD (n = 3). Statistical analysis was performed using an unpaired Student’s t test with a two-tailed distribution (* p < 0.05, **p < 0.01). Abbreviations: GPD1, glycerol-3-phosphate dehydrogenase 1; GSEA, Gene Set Enrichment Analysis; KEGG, Kyoto encyclopedia of genes and genomes; ccRCC, clear cell renal cell carcinoma; CCK-8, cell counting kit