Table 1.
S. No | Phyto constituents Analysed | W | WP |
WB |
WT |
WO |
WA |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
WP1 | WP2 | WP3 | WB1 | WB2 | WB3 | WT1 | WT2 | WT3 | WO1 | WO2 | WO3 | WA1 | WA2 | WA3 | |||
1. | Alkaloid test | ||||||||||||||||
1a. | Dragendroff | ++ | ++ | ++ | + | ++ | +++ | ++ | ++ | ++ | + | ++ | + | −− | ++ | ++ | + |
1b. | Mayer’s test | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− |
1c. | Wagner’s test | + | ++ | ++ | + | ++ | + | +++ | ++ | + | + | ++ | + | +++ | ++ | + | + |
2. | Tannins test | ||||||||||||||||
2a. | FeCl3 test | + | +++ | +++ | +++ | + | +++ | ++ | + | + | + | −− | + | ++ | −− | + | −− |
2b. | Lead sub acetate | −− | +++ | +++ | +++ | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− |
2c. | Gelatin test | −− | +++ | +++ | −− | + | +++ | ++ | + | −− | −− | −− | + | + | + | −− | −− |
3. | Flavonoids test | ||||||||||||||||
3a. | Ammonia | ++ | ++ | −− | ++ | ++ | −− | −− | ++ | +++ | ++ | + | −− | −− | + | −− | −− |
3b. | AlCl3 test | −− | ++ | +++ | + | ++ | +++ | ++ | ++ | +++ | ++ | + | +++ | −− | +++ | −− | −− |
4. | Carbohydrate test | ||||||||||||||||
4a. | Molisch test | ++ | ++ | +++ | ++ | +++ | +++ | +++ | + | ++ | + | +++ | +++ | +++ | +++ | +++ | ++ |
4b. | Fehling’s test | +++ | +++ | + | +++ | −− | −− | −− | ++ | +++ | ++ | ++ | + | ++ | ++ | +++ | ++ |
4c. | Benedict’s test | +++ | +++ | +++ | +++ | ++ | +++ | +++ | ++ | +++ | +++ | + | ++ | +++ | + | +++ | + |
5. | Cardiac glycoside | ||||||||||||||||
5a. | Keller-killiani | −− | ++ | +++ | + | −− | + | + | −− | −− | −− | −− | −− | +++ | + | +++ | ++ |
5b. | Legal test | +++ | +++ | +++ | +++ | +++ | ++ | ++ | ++ | + | + | + | + | +++ | ++ | ++ | −− |
6. | Steroids/Terpenes | ||||||||||||||||
6a. | Salkowski | + | ++ | +++ | + | +++ | + | +++ | ++ | ++ | ++ | + | +++ | +++ | ++ | +++ | ++ |
6b. | Liebermann Burch | ++ | −− | −− | + | + | + | ++ | + | + | ++ | + | + | +++ | + | ++ | + |
7. | Glycoside test | + | +++ | −− | ++ | −− | −− | −− | + | −− | −− | −− | + | ++ | −− | −− | −− |
8. | Anthraquinone | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− | −− |
9. | Saponins Frothing | ++ | −− | + | −− | ++ | +++ | +++ | + | −− | −− | −− | −− | −− | −− | ++ | +++ |
10. | Amino Ninhydrin | +++ | −− | −− | −− | +++ | −− | +++ | + | −− | + | −− | −− | +++ | +++ | −− | +++ |
11. | Protein Biuret | −− | ++ | +++ | ++ | ++ | ++ | ++ | + | ++ | + | + | +++ | +++ | −− | +++ | −− |
12. | Phenol Ellagic test | −− | ++ | +++ | +++ | −− | ++ | −− | −− | + | +++ | −− | + | ++ | −− | ++ | −− |
13. | Organic test | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
14. | Inorganic test | −− | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
15. | Coumarin | +++ | + | ++ | + | + | ++ | + | + | +++ | ++ | ++ | ++ | −− | +++ | +++ | + |
+++ High,++ Moderate,+ low, −− Absent; the experiments were conducted in triplicates, and classification was based on the intensity of color and amount of precipitate formed. W. somnifera (W) along with combination [4:1(1), 1:1(2), 1:4(3)] of P. emblica W:P (WP), B. monnieri W:B (WB), T. sinensis W:T (WT), O. basilicum W:O (WO), and A. racemosus W:A (WA).