Figure S2.
LN expansion requires input from blood and afferent lymph. (a) CFSE+ transferred T and B cells in popLN 1 wk after unilateral sciatic denervation; n = 8 mice from two independent experiments; unpaired Student’s t test. (b) Remaining fraction of adoptively transferred, labeled cells in intact or denervated popLNs after 18 h of homing blockade; n = 8 mice from two independent experiments. (c, d, and f) Counts of indicated subsets in popLNs 1 wk after unilateral sciatic denervation with or without (c) anti-αL/α4-integrin treatment (n = 3 or 4 mice; data are representative of two independent experiments), (d) anti–L-selectin treatment (n = 6 mice from two independent experiments), or (f) afferent lymphatic disconnection (n = 8–13 mice from three independent experiments); two-way ANOVA; Šídák’s post-test. (e) Expression of VCAM-1 on HEVs in sham-operated or denervated popLNs 2 wk after unilateral sciatic denervation; n = 5 mice; data are representative of two independent experiments; unpaired Student’s t test. All mice in this figure were 8–12-wk-old WT males purchased from Charles River or Janvier Labs. All data are presented as mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Mϕ, macrophage; MFI, mean fluorescence intensity; Sx, sciatic denervation.