Table 1.
Tested strains and plasmids used in this experiment
| Name | Properties and Application | Source |
|---|---|---|
| Strains | ||
| B. cereus 0–9 | Wild type strain in this study | Kept in our laboratory, isolated from wheat root. |
| E. coli 116 (pir+) | Plasmid propagation | Purchased from BioVector NTCC |
| E. coli GM2163 (dam-) | Demethylation | Purchased from BioVector NTCC |
| HQ1021 | The spoVG gene defect mutant, ∆spoVG | Construct in this study |
| ZY1001 | The abrB gene defect mutant, ∆abrB | Construct in this study |
| FPU1061I | The sinI gene defect mutant, ∆sinI | Construct in this study |
| FPU1062R | The sinR gene defect mutant, ∆sinR | Construct in this study |
| ZL1002 | The spo0A gene defect mutant, ∆spo0A | Construct in this study |
| HQ2021 | The double knockout strain of spoVG and sinI genes, ∆spoVG∆sinI | Construct in this study |
| HQ2022 | The double knockout strain of spoVG and sinR genes, ∆spoVG∆sinR | Construct in this study |
| HQ2023 | The double knockout strain of spoVG and abrB genes, ∆spoVG∆abrB | Construct in this study |
| HQ2024 | The double knockout strain of spoVG and spo0A genes, ∆spoVG∆spo0A | Construct in this study |
| HQ7110 | ΔspoVG supplemented with its native spoVG gene by allelic exchange, ∆spoVG::spoVG | Construct in this study |
| HQ7170 | ΔspoVG supplemented with spoVG gene from B. subtilis 168 by allelic exchange, ∆spoVG::spoVG 168 | Construct in this study |
| HQ7121 | HQ1021 supplemented with its native spo0A gene by reverse complementary, ∆spoVG/spo0A | Construct in this study |
| HQ7221 | HQ2024 supplemented with its native spo0A gene by reverse complementary, ∆spoVG∆spo0A/spo0A | Construct in this study |
| HQ7222 | HQ2024 supplemented with its native spoVG gene by reverse complementary, ∆spoVG∆spo0A/spoVG | Construct in this study |
| HQg6071 | B. cereus 0–9 with PexsY-GUS labeling | Construct in this study |
| HQg6171 | HQ1021 (ΔspoVG) with PexsY-GUS labeling | Construct in this study |
| HQg6072 | Wild B. cereus 0–9 with PsipW-GFP labeling | Construct in this study |
| HQg6172 | HQ1021 with PsipW-GFP labeling | Construct in this study |
| HQg6073 | Wild B. cereus 0–9 with PcalY-GFP labeling | Construct in this study |
| HQg6173 | HQ1021with PcalY-GFP labeling | Construct in this study |
| HQg6074 | Wild B. cereus 0–9 with PabrB-GFP labeling | Construct in this study |
| HQg6174 | HQ1021 with PabrB-GFP labeling | Construct in this study |
| HQg6175 | Wild B. cereus 0–9 with PsinI-GFP labeling | Construct in this study |
| HQg6175 | HQ1021 with PsinI-GFP labeling | Construct in this study |
| Plasmid | ||
| pAD | For gene knockout | Takara, Dalian |
| spoVG-pMAD | For gene knockout, Amp+; Erm+ | Construct in this study |
| abrB-pMAD | For gene knockout, Amp+; Erm+ | Construct in this study |
| pAD-pgal-JT | For reverse complementation | Stored in our laboratory |
| pMAD-chi | For gene complementation, Cm+ | Construct in this study |
| pET28a-gapB | Expression of protein, Km+ | Construct in this study |