Fig. 2.

Overexpression of ATF3 sensitized gastric cancer cells to cisplatin. A Cisplatin-resistant cells (SGC7901/DDP and BGC823/DDP) and their parental cells (SGC7901 and BGC823 cells) were exposed to various doses of cisplatin. Then, a CCK-8 assay was performed to determine cell viability. B, C mRNA levels of ATF3 in cisplatin-resistant and parental GC cells. D Western blotting was conducted to analyze protein expression of ATF3. E, F SGC7901/DDP and BGC823/DDP cells were transfected with recombinant ATF3 plasmids, and the effect on ATF3 protein expression was then evaluated by western blotting. G, H After transfection with rATF3 vectors, cell viability under cisplatin exposure was then detected. *P < 0.05. **P < 0.01