Fig. 6.

Nrf2/Keap1/xCT signaling involved in ATF3-mediated ferroptosis and cisplatin resistance in GC. A The effect of Nrf2 plasmid transfection on mRNA levels of Nrf2 was analyzed by qRT-PCR. B After transfection with Nrf2 vector, protein expression of Nrf2, Keap1 and xCT was measured. *P < 0.05, **P < 0.01 vs. vector group. C SGC7901/DDP cells were treated with ATF3 plasmids and/or Nrf2 plasmids before cisplatin exposure. Then, the levels of ROS (C), MDA (D), lipid peroxidation (E), GSH (F) and cell viability (G) were analyzed. *P < 0.05 vs. DDP group. ^§P < 0.05 vs. DDP and ATF3 group