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. 2021 Jun 8;21:300. doi: 10.1186/s12935-021-02006-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of OS malignancy on CD146 expression, including cycle migration and co-culture assay in vitro. A The proliferation of MG63 cells that come from the 0th, 9th or 15th time repeated migration was observed by MTT and Cell counting assays (n = 6). B The migration ability of MG63 cells, which were collected at the 0th, 9th, and 15th time migration, respectively, was measured by using Transwell system (n = 5). The migrated cells were counted by Image J software. The expression of CD146 mRNA (C) and protein (D) in MG63 cells derived from the 0th, 9th, and 15th time migration were detected by qPCR and Western blotting (n = 3). D In addition, 143B cells were co-cultured with MG63 cells for 0, 24 and 48 h to detect the CD146 expression, respectively (n = 3). Scale: × 200. **P < 0.01; ***P < 0.001