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Relationship between CD146 and the interaction of OS and HUVECs. A The sCD146 levels in the CM from single or co-culture system were measured. The protein expression of CD146 in OS (B) or HUVECs (C) were observed by Western blotting before and after OS cells were co-cultured with HUVECs. D The role of CD146 in heterotypic adhesion rates between them was detected. GAPDH was used as internal control. **P < 0.01, ***P < 0.001, n = 3
