Figure 5.
Effect of rTsP53 treatment on macrophages marker and cytokine expression in BALF macrophages from three groups. (a, b) Percentage of F4/80(+)/CD197(+) and F4/80(+)/CD206(+) macrophages was detected in control group. (c, d) Percentage of F4/80(+)/CD197(+) and F4/80(+)/CD206(+) macrophages was detected in LPS group at 6 h. (e, f) Percentage of F4/80(+)/CD197(+) and F4/80(+)/CD206(+) macrophages was detected in LPS + rTsP53 group at 6 h. (g, h) Percentage of F4/80(+)/CD197(+) and F4/80(+)/CD206(+) macrophages was detected in LPS group at 24 h. (i, j) Percentage of F4/80(+)/CD197(+) and F4/80(+)/CD206(+) macrophages was detected in LPS + rTsP53 group at 24 h. (k, l) the relative mRNA expression of TNF-α, IL-10, iNOS and Arg-1 was determined by RT-PCR in different groups at 24 h. Values are means ± SEM, *P < 0.005 vs. LPS group; #P < 0.01 vs. LPS 2 h+rTsP53 group. Data were expressed as the means ± SEM. *P < 0.05 vs. control group, #P < 0.05 vs. LPS group.