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. 2021 Jun 8;19:171. doi: 10.1186/s12951-021-00918-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — A The temperature changes of five different concentrations of USPIO-PEG-sLe^x nanoparticles under different power densities of NIR (a, b, and c) with a wavelength of 808 nm. B Cell viability of USPIO-PEG-sLe^x nanoparticles co-cultured with5-8F cells for 2 h, 4 h, 8 h, 24 h. C Cell viability of 5-8F cells in same concentration of USPIO-PEG-sLe^x nanoparticles at different culture times. D Cell viability of 5-8F cells with different concentrations USPIO-PEG-sLe^x nanoparticles in vitro PTT. E Trypan blue staining. Dead cells were stained blue, while living cells were translucent. The cell viability of each concentration was 80.00%, 75.21%, 78.33%, 75.80%, 69.45%, respectively (a, 0 mg/ml, b, 0.025 mg/ml, c, 0.05 mg/ml, d, 0.1 mg/ml, e, 0.2 mg/ml)