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. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Cancer Immunol Res. 2019 Feb 19;7(4):683–692. doi: 10.1158/2326-6066.CIR-18-0748

Figure 4.

Figure 4.

Expression of CD123.IL7Ra stimulates enhanced T-cell activation. A, Schematic of mutant IL7Rα receptors. B, STAT5 activation demonstrated downstream of IL7Rα receptor in engineered T cells. Cells were starved of cytokine, lysed, and Western blot analysis performed. Densitometry analysis calculated as p-STAT5/ STATS, (n = 1–2 donors of each T-cell type). C, Representative Western blot analysis for phospho-STAT5 and STAT5. Positive control (+): NT T cells treated with 10 ng/mL rhlL7 for 30 minutes prior to lysis.