KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-EBNA1 | Acris | Cat# BM3127 |
| Anti-Actin | Sigma | Cat# A3854 |
| Anti-Flag HRP | Sigma | Cat# A8592 |
| Anti-TOPO II | Abcam | Cat# ab52934 |
| Anti-PCNA | Abcam | Cat# ab92552 |
| Anti-Cyclin A | Invitrogen | Cat# PA5-99162 |
| Anti-Cyclin E | SCBT Inc | Cat# sc-377100 |
| Ati-CyclinB1 | Invitrogen | Cat# MA5-13128 |
| Anti-pH3S10 | Abcam | Cat# ab5176 |
| Anti-Ebna2 | Millipore | Cat# MABE8 |
| Anti-Lmp1 | Millipore | Cat# MABF2248 |
| Anti-Rpa32 | Abcam | Cat# ab7642 |
| Anti-EAD | Millipore | Cat# MAB8186 |
| Anti-IgG | SCBT Inc | Cat# sc-2025 |
| Anti-flag M2 | Sigma | Cat# F1804 |
| Anti-Trimethyl-histone H3 (Lys4) | Millipore | Cat# 07-473 |
| Anti-Rabbit IgG HRP | BioRad | Cat# 1706515 |
| Anti-Mouse IgG HRP | BioRad | Cat# 1706516 |
| Anti-Zta | In House | N/A |
| Bacterial and Virus Strains | ||
| Max Efficiency DH5a | Invitrogen | Cat# 128258-012 |
| E. coli strain GS1783 | Oesterdider/Cornell | N/A |
| E. coli strain DH10b | Oesterdider/Cornell | N/A |
| EBV B95-8 Bacmid | H.J.Delecluse/DKFZ | doi:10.1073/pnas.95.14.8245 |
| Biological Samples | ||
| ID#599 PBMC | Wistar | N/A |
| ID#180 PBMC | Wistar | N/A |
| ID#367 PBMC | Wistar | N/A |
| ID#296 PBMC | Wistar | N/A |
| ID# 323 PBMC | Wistar | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dulbecco’s Phosphate buffer saline(PBS) | Corning | 21-031-CM |
| Dulbecco’s Modified Eagle Medium (DMEM) | GIBCO | 2491-015 |
| RPMI Medium 1640 | GIBCO | 11875-085 |
| Fetal Bovine Serum | Hyclone | Cat# SH30396-03 |
| Trypsin | Corning | 25-053-CI |
| Penicillin/streptomycin | Corning | 30-002-CI |
| Primocin | InvivoGen | Cat# ant-pm05 |
| Lipofectamine 2000 | Invitrogen | 11668-019 |
| Hygromycin | Corning | 30-240-CR |
| Protease inhibitors | Sigma | P8340 |
| Phosphatase inhibitors | Roche | 04 906 837 001 |
| Aphidicolin | Sigma | A0781 |
| DTT | Sigma | D9779 |
| Gaunidine thiocyanate | Sigma | G5277 |
| Flag Magnetic beads | Sigma | M8823 |
| EBV-Ebna1 Agarose beads | SBCT | Cat# sc-81581AC |
| Protein A Sepharose | GE Healthcare | Cat# 17513801 |
| Protein G Seoharose | GE Healthcare | Cat#17061801 |
| His Trap FF | GE Healthcare | 17524802 |
| 3XFlag peptide | Sigma | F4799 |
| Tdp1 Human | Prospec | Cat# enz-685-b |
| Tdp2 Human | Prospec | Cat# enz-698-b |
| Proteinase K | Roche | 03115852001 |
| Critical Commercial Assays | ||
| B-cell enrichment Kit II | Stem Cell Tech | Cat#19054 |
| Bacmid purification kit | Clonetech | Cat# 740579 |
| Random Prime DNA labeling Kit | Tankara | Cat# 6045 |
| Custom TaqMan Gene Expression assays | Thermo-Fisher | ID APU667U |
| TaqMan Master Mix | Applied Biosystems | 4324018 |
| PureLink Plasmid Maxiprep Kit | Invitrogen | Cat#20220831 |
| Experimental Models: Cell Lines | ||
| Human: Raji | ATCC | Cat# CRL-7936 |
| Human: 293T | ATCC | Cat# CRL-11268 |
| Human: BCBL | Yan Yuan/UPENN | N/A |
| Marmoset B-cell line (B95-8) | ATCC | Cat# CRL-1612 |
| B95-8 LCL | This paper | N/A |
| Bac Wt LCL | This paper | N/A |
| BacY518F LCL | This paper | N/A |
| Oligonucleotides | ||
| 5’ tct gga tag cat atg cta tcc aga 3’ | DS (1 x binding site; self complementray used in Fig 3) | IDT |
| 5’ attaggatagcctatgctacccagatatagatta ggatagcatatgctacccagatatagagagcgtcacctaagcc 3’ 5’ggcttaggtgacgctctatatctgggtagcata tgctatcctaatctatatctgggtagcataggctatcctaat 3’ |
2X FR | IDT |
| 5’gcagctgacgcgtaattcgatagcatatgcttcccgttggcgctagc 3’ 5’ gctagcgccaacgggaagcatatgcttaggctaattccggactggtcg 3’ 5’ cgaccagtccggaattagccta 3 5’ atcgaattacgcgtcagctgc 3’ |
Y Arc | IDT |
| 5’attaggatagcctatgc 3’ 5’tacccagatatagattaggatagcatatgctacccagatatag 3’ FR3’top 5’ ctatatctgggtagcatatg 3’ 5’ctatcctaatctatatctgggtagcataggctatcctaat 3’ 3’bottom |
Nick DS | IDT |
| 5’ attaggatagcctatgctacccagatatagatta ggatagcatatgctacccagatatagag 3’ 5’ ctctatatctgggtagcatatgctatcctaatctaa attaggatagcatatactaccctatagc 3’ 5’ gctatagggtagtatatgctatcctaatttaaatat gggtagcctaatgcatccaactg 3’ 5’ cagttggatgcattaggctacccata tttatatctgggtagcataggctatcctaat 3’ |
4 WHJ | IDT |
| Recombinant DNA | ||
| pEP-KanS | Oesterdider/Cornell | N/A |
| pET-Duet Sumo EBNA1 DBD Wt | Juliana et al 2018 | N/A |
| pET-Duet Sumo EBNA1 DBD Y518F | This Paper | N/A |
| pET-Duet Sumo EBNA1 DBD Y518A | This Paper | N/A |
| pET-Duet Sumo EBNA1 DBD Y518E | This Paper | N/A |
| pET-Duet Sumo EBNA1 DBD H468A | This Paper | N/A |
| pET-Duet Sumo EBNA1 DBD N519A | This Paper | N/A |
| EBV Rep10 Flag EBNA 1 | Dheekollu et al 2017 | N/A |
| EBV Rep10 Flag Y518F | This Paper | N/A |
| EBV Rep10 Flag Y518A | This Paper | N/A |
| EBV Rep10 Flag H468A | This Paper | N/A |
| EBV Rep10 Flag H468A/Y518F | This Paper | N/A |
| EBV Bac95-8 | Delecluse HJ. | N/A |
| EBV Bac95-8 Y518F | This Paper | N/A |
| Software and Algorithms | ||
| NIS Elements AR | Nikon | Version 5. |
| ImageQuant TL | GE Healthcare | Version 8.2 |
| PRISM | Graph Pad Software | Version 6 |
| Pymol | Schrodinger | https://www.pymol.org/ |
| WINMDI software | Scripps Institute | N/A |
| Other | ||
| Enzymes | ||
| P1 Nuclease | NEB | Cat#M0660S |
| Exonuclease VII | NEB | Cat# M0379S |
| RecJF | NEB | Cat# M0264S |
| Mung bean nuclease | NEB | Cat# M0250S |
| T4PNK | NEB | Cat# M0201S |
| DpnI | NEB | R0176L |
| BamHI | NEB | R3136L |
| Dra1 | NEB | R0129L |
| DNase I | Roche | Cat# 04-716728-001 |
| RNaseA | Invitrogen | Cat# 12091-021 |
| Benzonase | Millipore | Cat# 71206-3 |