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. 2021 May 25;12:663041. doi: 10.3389/fimmu.2021.663041

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Copyright © 2021 Pearson, Tedla, Becker, Nakajima, Jasinskas, Mduluza, Mutapi, Oeuvray, Greco, Sotillo, Felgner and Loukas

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quality control probe of the S. haematobium protein microarray. Arrays were probed with (A) anti-hemagglutinin (HA) antibody to detect expression of the N-terminal HA tag and determine the percentage of protein expression from all arrayed antigens, and (B) anti-6-HIS antibody to detect expression of the C-terminal 6-HIS tag and determine the percentage of full-length protein expression from all arrayed antigens. No DNA refers to control spots where plasmid DNA (encoding genes to be expressed) is omitted from the reverse transcription-translational products printed at those locations. Sh Adult ES, S. haematobium adult fluke Excretory/Secretory products; Sh Adult PBS extract, S. haematobium adult fluke PBS-soluble somatic extract; Sh egg ES, S. haematobium egg ES products; Sh Adult triton extract, S. haematobium adult fluke TritonX-114-soluble extract; Sh SEA, S. haematobium soluble egg antigen.