| Antibodies |
|
|
|
| Brilliant Violet 510 anti-mouse Ly-6G Antibody, clone 1A8 |
BioLegend |
RRID:AB_2562937; Cat#127633 |
| PE/Cyanine7 anti-mouse Ly-6C Antibody, clone HK1.4 |
BioLegend |
RRID:AB_1732082; Cat#128018 |
| FITC anti-mouse F4/80 Antibody, clone BM8 |
BioLegend |
RRID:AB_893502; Cat#123108 |
| PE anti-mouse IL-1α Antibody, clone ALF-161 |
BioLegend |
Cat#503203 |
| IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), APC |
ThermoFisher Scientific |
RRID:AB_10670739; Cat#17-7114-80 |
| Fixable Viability Dye eFluor 780 |
eBioscience |
Cat#65-0865-18 |
| Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate |
Invitrogen |
Cat#W11261
|
| FITC anti-mouse Ly-6G Antibody, clone 1A8 |
BioLegend |
RRID:AB_1236494; Cat#127606 |
| PE anti-mouse Ly-6G Antibody, clone 1A8 |
BioLegend |
RRID:AB_1186099; Cat#127608 |
| Brilliant Violet 605 anti-mouse CD11c Antibody, clone N418 |
BioLegend |
RRID:AB_2562415; Cat#117334 |
| PE anti-mouse F4/80 Antibody, clone BM8 |
BioLegend |
RRID:AB_893486; Cat#123110 |
| PE anti-mouse/human CD11b Antibody, clone M1/70 |
BioLegend |
RRID:AB_312791; Cat#101208 |
| APC anti-mouse CD63 Antibody, clone NVG-2 |
BioLegend |
RRID:AB_2565496; Cat#143906 |
| APC anti-mouse CD9 Antibody, clone MZ3 |
BioLegend |
RRID:AB_2783070; Cat#124812 |
| Recombinant Anti-GSDMD antibody [EPR20859] |
Abcam |
RRID:AB_2888940; Cat#ab219800 |
| Actin Antibody (C-2) |
Santa Cruz Biotechnology |
RRID:AB_626630; Cat#sc-8432 |
| Mouse IL-1 beta/IL-1F2 Antibody |
R&D Systems |
RRID:AB_354347; Cat#AF-401-NA |
| Anti-mouse IgG, HRP-linked Antibody |
Cell Signaling Technology |
RRID:AB_330924; Cat#7076S |
| Anti-rabbit IgG, HRP-linked Antibody |
Cell Signaling Technology |
RRID:AB_2099233; Cat#7074S |
| IL-1α Hamster anti-Mouse, PE, Clone: ALF-161, BD |
Fisher Scientific |
Cat#BDB559810 |
| Recombinant Anti-CD63 antibody [EPR21151] |
Abcam |
Cat#ab217345 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 |
ThermoFisher Scientific |
RRID:AB_2534093; Cat#A-11035 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 |
ThermoFisher Scientific |
RRID:AB_2536183; Cat#A-31573 |
|
| Bacterial and virus strains |
|
|
|
|
Aspergillus fumigatus, Strain: CEA10 |
Dr. Robert Cramer, Dartmouth, New Hampshire |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Casein sodium salt from bovine milk |
Sigma Aldrich |
CAS#9005-46-3; Cat#C8654-500G |
| Mouse Recombinant GM-CSF (E. coli-expressed) |
StemCell Technologies, Inc. |
Cat#78017.1 |
| Curdlan from Alcaligenes faecalis
|
Sigma Aldrich |
CAS#54724-00-4; Cat#C7821-5G |
| LPS-EK ULTRAPURE (Ultrapure lipopolysaccharide from E. coli K12) |
InvivoGen |
Cat#tlrl-peklps |
| Adenosine 5′-triphosphate disodium salt hydratemicrobial, BioReagent, suitable for cell culture, ≥ 99% (HPLC) |
Sigma Aldrich |
CAS#34369-07-8; Cat#A6419-1G |
| Normal Donkey Serum |
Jackson Immunoresearch |
RRID:AB_2337258; Cat#017-000-121 |
| Regular Fetal Bovine Serum, Heat Inactivated |
Corning |
Cat#MT35011CV |
| VECTASHIELD® HardSet Antifade Mounting Medium with DAPI |
Vector Laboratories |
Cat#H-1500-10 |
| QUANTI-Blue™ Solution (Alkaline phosphatase detection medium - Liquid form) |
InvivoGen |
Cat#rep-qbs2 |
| Propidium iodide, 1mg/ml aqueous soln. |
Alfa Aesar |
CAS#25535-16-4; Cat#J66584-AB |
| Exosome-depleted FBS Media Supplement |
System Biosciences (SBI) |
Cat#EXO-FBS-250A-1 |
| ExoQuick-TC |
System Biosciences (SBI) |
Cat#EXOTC10A-1 |
| Diisopropylfluorophosphate (DFP) |
Sigma Aldrich |
CAS#55-91-4; Cat#D0879-1G |
| Cell Lysis Buffer (10X) |
Cell Signaling Technology |
Cat#9803S |
| HEK-Blue™ Selection (Antibiotics for maintenance of HEK-Blue Cells) |
InvivoGen |
Cat#hb-sel |
|
| Critical commercial assays |
|
|
|
| EasySep™ Mouse Neutrophil Enrichment Kit |
StemCell Technologies, Inc. |
Cat#19762 |
| Pierce BCA Protein Assay Kit - Reducing Agent Compatible |
ThermoFisher Scientific |
Cat#23250 |
| SuperSignal West Femto Maximum Sensitivity Substrate |
ThermoFisher Scientific |
Cat#34095 |
| Mouse IL-1 alpha/IL-1F1 Duoset ELISA |
R&D Systems |
Cat#DY400-05 |
| Mouse IL-1 beta/IL-1F2 Duoset ELISA |
R&D Systems |
Cat#DY401 -05 |
| CytoTox 96® Non-Radioactive Cytotoxicity Assay |
Promega |
Cat#G1780 |
| BD Cytofix/Cytoperm Fixation/Permeablization Kit |
BD Biosciences |
RRID:AB_2869008; Cat#554714 |
|
| Experimental models: Cell lines |
|
|
|
| HEK-Blue™ IL-1R Cells (HEK293 reporter cells for human and murine IL-1α & IL-1β cytokines) |
InvivoGen |
Cat#hkb-il1r |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: C57BL/6J |
The Jackson Laboratory (JAX) |
Cat#000664 |
| Mouse: GSDMD−/−, C57BL/6J background |
Dr. Russel Vance, University of California, Berkeley |
N/A |
| Mouse: NLRP3−/−, C57BL/6J background |
Millennium Pharmaceuticals, Cambridge, MA |
N/A |
| Mouse: IL-1α−/−, C57BL/6J background |
Dr. Y. Iwakura, University of Tokyo |
N/A |
| Mouse: IL-1β−/−, C57BL/6J background |
Dr. Y. Iwakura, University of Tokyo |
N/A |
| Mouse: IL-1α−/− IL-1β−/−, C57BL/6J background |
Dr. Y. Iwakura, University of Tokyo |
N/A |
|
| Software and algorithms |
|
|
|
| Flowjo |
Flowjo |
RRID: SCR_008520 |
| GraphPad Prism |
GraphPad |
RRID:SCR_002798 |
| IDEAS |
AMNIS, Luminex |
N/A |
| NovoExpress Software |
Agilent |
N/A |
