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. 2021 May 25;12:659210. doi: 10.3389/fmicb.2021.659210

FIGURE 1.

FIGURE 1

Infection of CHV1 and MyRV1 in V. mali. (A) Agarose gel electrophoresis of dsRNAs extracted from V. mali strains singly or doubly infected with CHV1 and MyRV1. The dsRNA samples were analyzed via electrophoresis in an agarose gel stained with ethidium bromide. The CHV1 dsRNA bands that migrated slightly faster than the wild-type CHV1 are defective interference RNA (DI-RNA). (B) Phenotypic growth on PDA medium of representative V. mali strains singly or doubly infected with CHV1 and MyRV1. The colonies were grown on PDA for 4 days and then photographed. (C) The growth areas of fungal strains described in (B). The data are the means ± SD (n = 3). The different letters indicate a significant difference at p < 0.01 (one-way ANOVA). (D) Representative images showing the formation of asexual fruiting bodies (pycnidia) of the fungal strains described in (B). The fungi were cultured on PDA medium for 4–5 weeks until the pycnidia were produced. Bars equal 1 cm. (E) The number of pycnidia counted from the fungal strains described in (D). The data are the means ± SD (n = 3). The different letters indicate a significant difference at p < 0.01 (one-way ANOVA).