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The relative transcript expression of RNA silencing-related genes in V. mali strains singly or doubly infected with CHV1 and MyRV1. The transcript expression of V. mali dcl1, dcl2, agl1, agl2, and agl3 genes following single and double virus infection were analyzed by quantitative RT-PCR. The data are the means ± SD (n = 3). The different letters indicate a significant difference at p < 0.01 (one-way ANOVA).
