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. 2021 Jun 2;109(11):1825–1835.e5. doi: 10.1016/j.neuron.2021.03.038

Figure 3.

Figure 3

Expression of uN2CpolyG is toxic in cell culture

(A) GFP fluorescence and immunofluorescence against p62 and Map2 of cortical neuronal cell cultures from mouse embryos transduced for 24 h with GFP, uN2C-GFP (12 GGC), uN2CpolyG-GFP (100 GGC), or ATG polyG-GFP (70 GGC) AAV2/PHP.eB. Scale bars, 10 μm. Nuclei were counterstained with DAPI.

(B) Immunoblot against GFP or the GAPDH of proteins extracted from uN2CpolyG-GFP-transfected HEK293 cells treated for 15 h with the indicated drugs.

(C) Cell viability of cortical neuronal cell cultures from mouse embryos transduced with GFP, uN2C-GFP, uN2CpolyG-GFP, or ATG polyG-GFP AAV2/PHP.eB. Error bars indicate SEM. Student’s t test, ∗∗∗p < 0.001.

See also Figures S3, S4, and Table S2.