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. 2021 Jun 8;16(6):e0252424. doi: 10.1371/journal.pone.0252424

Fig 4. Role of myeloid cells on gel contraction.

Fig 4

A) FACS plots (DAPI-) before and after isolation of CD11b+ cells from s.c. LLC tumor tissue using CD11b magnetic microbeads. Gate (CD45+CD11b+) indicates average purity of isolated cells based on 5 separate experiments. B) Representative gel pictures after performing gel contraction assay. C) Gel contraction assay of immortalized mouse mammary fibroblasts co-incubated with CD11b+ cells. Graphs show gel area, mm2. *—p<0.05 vs. TGFβ group. Primary mouse mammary fibroblast isolated from mouse mammary gland WT animals by FACS sorting as CD104a+ (CD45-CD326-) cells. Cells were used in passage 1. CD11b cells were isolated from LLC implanted tumors by magnetic microbeads. Fibroblasts and CD11b cells were embedded to collagen gel and cultured 24 hr. TGFβ (1 ng/ml) and AMP/ATP (100 uM) was added for 6 hr. with following measurement of gel diameter.