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. 2021 Jun 8;16(6):e0252822. doi: 10.1371/journal.pone.0252822

Fig 1. Growth inhibitory effects of tamoxifen and expression of ERα, PgR and HER2 in ER-positive breast cancer cell lines and their tamoxifen-resistant sublines.

Fig 1

The growth inhibitory effects of TAM in wt-MCF7, MCF7/TAM, wt-T47D, T47D/T, BT474, and BT474/T was evaluated by WST assays (A). Closed circles (●) indicate wild-type cells, whereas closed squares (■) indicate TAM-resistant sublines. The error bars represent the standard errors of the values obtained from triplicate experiments. (B) ERα, PgR, and HER2 protein expression levels were analyzed by western blotting. β-actin was used as a loading control. Each experiment was independently performed and repeated at least three times, and one representative result is provided in the figures.