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. 2021 Jun 8;16(6):e0252822. doi: 10.1371/journal.pone.0252822

Fig 3. Apoptosis induced by chemotherapeutic agents in ER-positive breast cancer cell lines and their tamoxifen-resistant sublines.

Fig 3

The effect of 5-fluorouracil (A), paclitaxel (B), and doxorubicin (C) on apoptosis in wild-type and TAM-resistant MCF7, T47D, and BT474 cells was examined. The Cell Death Detection ELISA plus kit was used to quantify apoptosis in the presence of 5-fluorouracil (50 μM–2 mM), paclitaxel (5 nM–1 μM), or doxorubicin (150 nM–700 μM) for 48 h. The error bars represent the standard deviations of the values obtained; each experiment was performed in duplicate. The experiments were repeated independently at least three times, and one representative result is provided in the figures. NS, not significant; *p < 0.05, unpaired Student’s t-tests.