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. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Arch Biochem Biophys. 2018 Dec 17;662:177–189. doi: 10.1016/j.abb.2018.12.019

Figure 4. Long-chain length polyphosphate but not short-chain length polyphosphate enhances ADP-dependent respiration and mitochondrial respiratory control ratio.

Figure 4.

(A) Oxygen consumption in isolated mitochondria from WT mouse hearts at baseline (basal) and upon subsequent addition of the mitochondrial complex I substrates (5 mmol/L malate and 5 mmol/L glutamate) in the presence of 2 µM free Ca2+ to induce State 2 of respiration followed by addition of 1 mmol/L ADP to induce State 3 of respiration in the absence or presence of 100 µM short or long chain polyPs. (B) Oxygen consumption in isolated mitochondria from CypD KO mouse hearts at baseline (basal) and upon subsequent addition of the mitochondrial complex I substrates (5 mmol/L malate and 5 mmol/L glutamate) in the presence of 2 µM free Ca2+ to induce State 2 of respiration followed by addition of 1 mmol/L ADP to induce State 3 of respiration in the absence or presence of 100 µM short or long chain polyPs. (C) Respiratory control ratio (RCR) was decreased in mitochondria treated with short polyPs in CypD-dependent manner while long chain polyPs increased RCR independently of CypD presence. n=mitochondrial preparations from 3–5 WT and CypD KO animals. Data expressed as mean±SEM. *p<0.05 vs corresponding no polyP conditions.