Figure 7. mPTP opening and ROS generation was observed in mouse cardiac myocytes exposed to simulated chemical ischemia and reperfusion.

(A) mPTP activity monitored by changes in calcein fluorescence during 20 min ischemia and 15 min of reperfusion in control wild type cardiac myocytes in the absence (WT) or presence of 1 µM of CsA (WT + CsA), and myocytes from cyclophilin D knockout (CypD KO) mice. (B) Summary of the mPTP activity during ischemia and reperfusion calculated as the rate of calcein release from mitochondria normalized to the basal rate. (C) Superoxide generation during ischemia and reperfusion in 3 experimental groups (WT, WT + CsA, and CypD KO) calculated as the rate of MitoSOX Red fluorescence increase relative the basal rate. Data expressed as mean±SEM. n=number of cells from 3 to 5 WT and CypD KO animals. * P<0.05 between no treatment (basal level) vs ischemia and ischemia-reperfusion, ** P<0.05 between ischemia vs ischemia-reperfusion, # P<0.05 between WT vs WT +CsA and CypD KO