Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (D. mel) | Schneider 2 (S2) cells | CCTCC (China Center for Type Culture Collection) | Serial# GDC0138 | Cell species report and Mycoplasma contamination test reports provided |
| Antibody | Rabbit anti-αNOMPC-EC (polyclonoal) | Ref. (Zhang et al., 2015) | Immunostaining dilution (1: 500), primary antibody | |
| Antibody | Alexa Fluor 594 AffiniPure Donkey Anti Rabbit IgG(H + L) | Yeason | Cat# 34212ES60 | Immunostaining dilution (1: 100), secondary antibody |
| Recombinant DNA reagent | pActin-Gal4 (plasmid) | Ref. (Yan et al., 2013) | Plasmid for driving Gal4 expression under actin promoter in S2 cells | |
| Recombinant DNA reagent | pUAST-NOMPC-EGFP (plasmid) | Ref. (Yan et al., 2013) | Plamid for Gal4-driven NompC expression in S2 cells | |
| Recombinant DNA reagent | pUAST-NOMPC-EGFP (del-miniwhite,dm) (plasmid) | This paper | Plamid for Gal4-driven WT NompC expression in S2 cells, no miniwhite sequence | |
| Recombinant DNA reagent | pUAST-NOMPC(D1236A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on D1236 | |
| Recombinant DNA reagent | pUAST-NOMPC(R1581A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on R1581 | |
| Recombinant DNA reagent | pUAST-NOMPC(K1244A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on K1244 | |
| Recombinant DNA reagent | pUAST-NOMPC(E1571A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on E1571 | |
| Recombinant DNA reagent | pUAST-NOMPC(Q1253A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on Q1253 | |
| Recombinant DNA reagent | pUAST-NOMPC(S1577A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on S1577 | |
| Recombinant DNA reagent | pUAST-NOMPC(S1421A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on S1421 | |
| Recombinant DNA reagent | pUAST-NOMPC(W1572A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on W1572 | |
| Recombinant DNA reagent | pUAST-NOMPC(W1115A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on W1115 | |
| Recombinant DNA reagent | pUAST-NOMPC(D1142A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on D1142 | |
| Recombinant DNA reagent | pUAST-NOMPC(R1127A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on R1127 | |
| Recombinant DNA reagent | pUAST-NOMPC(E1163A)-EGFP(dm) (plasmid) | This paper | Contains Drosophila NOMPC CDS with alanine substitution on E1163 | |
| Chemical compound, drug | 1-Oleoyl-2-acetyl-sn-glycerol (OAG) | Sigma-Aldrich | Cat# O6754 | DAG analogue |
| Chemical compound, drug | GdCl3 | Sigma-Aldrich | Cat# 439770 | NOMPC blocker |
| Chemical compound, drug | Concanavalin A (Con A) | Sigma-Aldrich | Cat# C5275 | Cell adhesion |
| Chemical compound, drug | ClonExpress II One-step Cloning Kit | Vazyme | Serial# C112 | Site-directed mutagenesis |
| Chemical compound, drug | TransIT-Insect Transfection Reagent | Mirus | Cat# MIR 6100 | S2 cell transfection |