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. 2021 Jun 9;190:113421. doi: 10.1016/j.bios.2021.113421

Fig. 5.

Fig. 5

Specificity and reproducibility of the SERS immunoassay. (A, B) The SERS spectra from the sandwich specificity immunoassay of SARS-CoV-2 spike protein, HIV-1 p24, EBV GP350, CEA, AFP and BSA and corresponding peak intensities at 1077 cm−1. The concentration of target antigen is 0.1 ng mL−1 while the other proteins are 1 μg mL−1. (C) Reproducibility of SERS spectra of sandwich immunoassay structure. (D) Relative standard deviation (RSD) of the peak intensity at 1077 cm−1 and 1586 cm−1, respectively. The spectra were collected from different points of sandwich immunoassay structure.