Survival after heat stress. Nematodes at the L4 stage were treated with nine different RNAi strains or the EV strain. On the third, seventh and twelfth day of adulthood, they were exposed to heat stress at 37°C for 3 h, prior to monitoring their survival. The mean survival ± s.e.m. is plotted (a,b) and differences compared to control were considered significant at p < 0.05 (*) by using a two-sided log-rank test and subsequent Bonferroni correction. In addition, survival curves are shown for nematodes which were heat shocked on the twelfth day of adulthood (c,d). The number of tested individuals is collected in table 1.