Table 1.
Heat stress survival characteristics during RNAi treatments. Differences compared to control were considered significant at p < 0.05 (*). p-value determination was realized with log-rank test and subsequent Bonferroni correction for the mean survival and Fisher's Exact test for specific time points. n = number of worms; min./med./max. = minimum/median/maximum; s.e.m. = standard error of the mean.
| days until deaths of population reached |
||||||||
|---|---|---|---|---|---|---|---|---|
| age at heat stress exposure | RNAi treatment | n | 25% (min. survival) | 50% (med. survival) | 90% (max. survival) | mean survival (days) | s.e.m. | change relative to control [%] |
| third day of adulthood | EV | 157 | 2.38 | 3.48 | 5.55 | 3.96 | 0.13 | |
| acox-1.1 | 151 | 1.90* | 2.87* | 5.17 | 3.49 | 0.13 | −13.47 | |
| daf-22 | 152 | 1.93 | 3.21 | 4.86 | 3.52 | 0.12 | −12.50 | |
| cat-4 | 152 | 1.43* | 2.41* | 4.65 | 3.05* | 0.12 | −29.84 | |
| pept-1 | 151 | 2.28 | 3.38 | 5.22 | 3.84 | 0.12 | −3.13 | |
| EV | 244 | 4.05 | 4.77 | 8.28 | 5.85 | 0.13 | ||
| pig-1 | 249 | 3.43* | 5.05 | 7.48* | 5.47 | 0.12 | −6.95 | |
| pak-2 | 240 | 4.26* | 5.21* | 8.57 | 6.24 | 0.13 | +6.25 | |
| sad-1 | 243 | 3.85 | 4.69 | 7.59 | 5.59 | 0.12 | −4.65 | |
| gsk-3 | 244 | 3.89 | 5.32* | 8.46 | 6.15 | 0.13 | +4.88 | |
| C25G6.3 | 241 | 3.74* | 4.74 | 7.74 | 5.69 | 0.11 | −2.81 | |
| seventh day of adulthood | EV | 158 | 1.81 | 2.73 | 4.34 | 3.23 | 0.09 | |
| acox-1.1 | 153 | 1.26* | 1.88* | 3.47* | 2.51* | 0.08 | −28.69 | |
| daf-22 | 156 | 1.43* | 2.13* | 3.97 | 2.78* | 0.09 | −16.19 | |
| cat-4 | 158 | 1.35* | 2.11* | 3.95 | 2.80* | 0.10 | −15.36 | |
| pept-1 | 156 | 1.65 | 2.41* | 3.98 | 3.03 | 0.09 | −6.60 | |
| EV | 228 | 2.60 | 4.05 | 6.67 | 4.61 | 0.12 | ||
| pig-1 | 263 | 1.87* | 2.90* | 5.47* | 3.61* | 0.10 | −27.70 | |
| pak-2 | 255 | 2.23 | 3.73 | 6.61 | 4.39 | 0.13 | −5.01 | |
| sad-1 | 253 | 2.54 | 4.16 | 6.71 | 4.63 | 0.12 | +0.43 | |
| gsk-3 | 246 | 1.77* | 2.84* | 6.22 | 3.84* | 0.12 | −20.05 | |
| C25G6.3 | 238 | 2.04* | 3.16* | 6.41 | 4.08 | 0.12 | −12.99 | |
| twelfth day of adulthood | EV | 144 | 1.34 | 2.54 | 4.98 | 3.22 | 0.14 | |
| acox-1.1 | 150 | 1.22 | 2.31 | 4.48 | 2.93 | 0.12 | −9.90 | |
| daf-22 | 154 | 0.40* | 0.80* | 2.60* | 1.60* | 0.08 | −101.25 | |
| cat-4 | 157 | 0.93* | 1.89 | 4.81 | 2.83 | 0.14 | −13.78 | |
| pept-1 | 159 | 1.02* | 1.93 | 4.95 | 2.91 | 0.14 | −10.65 | |
| EV | 193 | 1.63 | 2.65 | 5.89 | 3.50 | 0.13 | ||
| pig-1 | 224 | 1.49* | 2.30 | 4.17* | 3.04* | 0.08 | −15.13 | |
| pak-2 | 178 | 1.16* | 1.89* | 4.42 | 2.75* | 0.12 | −27.27 | |
| sad-1 | 186 | 1.39 | 2.46 | 5.28 | 3.21 | 0.13 | −9.03 | |
| gsk-3 | 155 | 0.41* | 0.82* | 2.32* | 1.57* | 0.07 | −122.93 | |
| C25G6.3 | 169 | 1.39* | 2.18* | 4.39* | 2.91* | 0.11 | −20.27 | |