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Sensitivity of the Sanger sequencing assay for the TERTp mutation. The percentage of C228T mutants in genomic DNA from mutant and wild-type cell lines (U251: C228T; OL: WT) is 5% (a), 10% (b), 15% (c), 20% (d), 30% (e), and 50% (f). TERTp: the promoter region of the telomerase reverse transcriptase gene.
