Table 1.
Primer sequences per gene.
| Genes | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplified fragments |
|---|---|---|---|
| TERT | M13F-AGTGGATTCGCGGGCACAGA | M13R-CAGCGCTGCCTGAAACTC | 235 bp |
| IDH1 | M13F-GAGAAGAGGGTTGAGGAGTT | M13R-TACCTTGCTTAATGGGTGTA | 294 bp |
| IDH2 | M13F-GCTTGGGGTTCAAATTCTG | M13R-CGGTCTGCCACAAAGTCTG | 343 bp |
| M13-tagged primer | TGTAAAACGACGGCCAGT | CAGGAAACAGCTATGACC | / |
| MSP-M | TTTCGACGTTCGTAGGTTTTCGC | GCACTCTTCCGAAAACGAAACG | 81 bp |
| MSP-U | TTTGTGTTTTGATGTTTGTAGGTTTTTGT | AACTCCACACTCTTCCAAAAACAAAACA | 93 bp |
TERT: telomerase reverse transcriptase; IDH1: isocitrate dehydrogenase 1; IDH2: isocitrate dehydrogenase 2; M13F: M13 universal forward sequence-tagged primers; M13R: M13 universal reverse sequence-tagged primers. MSP-M: methylation special PCR methylated primers; MSP-U: methylation special PCR unmethylated primers.