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Precision of the Sanger sequencing assay for TERTp mutations. Performance of interassay and intra-assay coefficients of variation.
| Concentration | n | Total | Within-day | Between-day | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | CV (%) | Mean | SD | CV (%) | Mean | SD | CV (%) | ||
| 50% | 30 | 1.14 | 0.12 | 10.72 | 1.21 | 0.02 | 1.84 | 1.20 | 0.12 | 10.00 |
| 15% | 30 | 7.14 | 0.93 | 13.02 | 5.67 | 0.13 | 2.26 | 6.61 | 0.71 | 10.73 |
TERTp: the promoter region of the telomerase reverse transcriptase gene; SD: standard deviation; CV: coefficient of variation.
