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. 2021 Jun 1;2021:8032125. doi: 10.1155/2021/8032125

Figure 2.

Figure 2

Isolation and identification of CBP22. (a) Cation exchange chromatography of the supernatant from C. butyricum culture medium at a wavelength of 215 nm and SDS-PAGE analysis. M: mark; lane 1: before purification; lane 2: after purification. (b) Affinity chromatography of products from (a) at a wavelength of 215 nm and SDS-PAGE analysis. M: mark; lane 1: before purification; lane 2: after purification. (c) Secondary structure prediction of CBP22. (d) Helical wheel plots of CBP22. (e) Hemolytic activity of CBP22 against porcine red blood cells. The hemoglobin release was monitored at 576 nm. The data are expressed as the mean ± SEM, n = 3 biological replicates; bars with different small capital letters are statistically different from one another.