Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Specific primers used for real-time PCR.
| Primer | Sequence (5′–3′) | Length (bp) | Tm (°C) | |
|---|---|---|---|---|
| ZO-1 | F | TCATCCCAAATAAGAACAGAGC | 198 | 60 |
| R | GAAGAACAACCCTTTCATAAGC | |||
|
| ||||
| ZO-2 | F | GCTTTGGTGTGGACCAAGAT | 269 | 60 |
| R | TCCATTATGGGTTTGCATGA | |||
|
| ||||
| Claudin-1 | F | GCTGGGTTTCATCCTGGCTTCT | 110 | 60 |
| R | CCTGAGCGGTCACGATGTTGTC | |||
|
| ||||
| Occludin | F | AAGCAAGTGAAGGGATCTGC | 204 | 60 |
| R | GGGGTTATGGTCCAAAGTCA | |||
| TNF-α | F | GCATGGTGGTGGTTGTTTCTGACGAT | 99 | 60 |
| R | GCTTCTGTTGGACACCTGGAGACA | |||
|
| ||||
| GAPDH | F | CAACGGCACAGTCAAGGCTGAGA | 112 | 60 |
| R | CTCAGCACCAGCATCACCCCAT | |||
