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. 2021 May 31;2021:1728352. doi: 10.1155/2021/1728352

Figure 5.

Figure 5

H19 is a direct transcriptional target of CREB1. (a) The expression of CREB1 in T98G cells at different time points after irradiation. (b) Clonogenic survival assay of T98G cells after transfection with siRNA-ctrl or siRNA-CREB1 and irradiation with X-rays (n = 3). (c) Real-time PCR analysis of H19 expression in T98G cells transfection with siRNA-ctrl or siRNA-CREB1 and irradiation with X-rays (n = 3). (d) Schematic illustration of firefly luciferase reporter plasmids. (e, f) Relative luciferase activity in 293T cells co-transfected with the indicated reporter constructs and Renilla luciferase plasmid (n = 3). Lenti is the vector of OE-CREB1 and pGL3 is the vector of H19 Promoter. ∗p < 0.05; ∗∗p < 0.01 compared with the control group.