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. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Cancer Cell. 2020 May 21;37(6):834–849.e13. doi: 10.1016/j.ccell.2020.04.014

Figure 4. G9a/GLP Mediate SETD5com Methylation Activity.

Figure 4.

(A) Generation of G9a/GLP-depleted 293T cells. Western analysis with the indicated antibodies of control (293T) or G9a/GLP co-depleted 293T cell lysates (293TDKD). Tubulin is shown as a loading control.

(B) Methylation of H3K9 by SETD5com requires G9a and GLP. Western analysis with the indicated antibodies of in vitro methylation assay as in Figure 3D using SETD5com purified from 293T or 293TDKD cells.

(C) Methylation of H3K9 by SETDcom is inhibited by the G9a/GLP inhibitor (UNC0638, G9ai). Western analysis with the indicated antibodies of in vitro methylation assay on recombinant nucleosomes with G9a and SETD5com ± UNC0638 (2 μM).