Table 2. Preparation of 10× pre-mix for a 50 µl inner reaction.

Component	Stock concentration	Final concentration in reaction (1×)	Final concentration (10×)	Volume
rNTP	25 mM	0.8 mM	8 mM	19.2 µl
HEPES, pH 7.58	2 M	55 mM	550 mM	16.5 µl
Folinic acid	10 mM	68 µM	680 µM	4.08 µl
cAMP	100 mM	0.64 mM	6.4 mM	3.84 µl
NH4OAc	10 M	27.5 mM	275 mM	1.65 µl
MilliQ water	-	-	-	Fill up to 60 µl