Fig. 4.

48h growth assay of RH-GFP tachyzoites. Microtiter plates with the compounds were infected with an inoculum of approximately 10,000 parasites per well. Molecules 1B, 2B, 3B, 4B, 6B and 12B reduce the percentage of growth similarly to pyrimethamine. Fluorescence of GFP RH parasites was quantified by fluorescent microscopy after 48h of treatment by using a standard fluorescence EVOS Light Cube GFP. Excitation 470/22 Emission: 526/60 with a 10× objective on EVOS FL Color Imaging System. Images were recorded with and adjusted for contrast with ImageJ.