Fig. 5.

Cerebral microvascular endothelial cells exposed to sEVs obtained from hypoxic MSCs exhibit a distinct microRNA signature associated with angiogenesis. Total counts of A miR-126-3p, B miR-140-5p, C let-7c-5p, D miR-186-5p, E miR-370-3p and F miR-409-3p, evaluated by NanoString analysis, in hCMEC/D3 cells exposed to control conditions, sEVs obtained from MSC culture media that contain platelet lysates (50 µg/mL; sEV_platelet), sEVs obtained from MSCs (

source 41.5) cultured under regular ‘normoxic’ conditions (21% O₂; 50 µg/mL; sEV_normoxic) or sEVs obtained from MSCs (source 41.5) cultured under hypoxic conditions (1% O₂; 50 µg/mL; sEV_hypoxic). Data are box plots with medians (lines inside boxes)/means (crosses inside boxes) ± interquartile ranges (IQR; boxes) with minimum/maximum values as whiskers (n = 4–6 samples per group). *p < 0.05, **p < 0.01, ***p < 0.001 compared with control/^††p < 0.01, ^†††p < 0.001 compared with sEV_platelet/^‡p < 0.05, ^‡‡p < 0.01 compared with sEV_normoxic